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Blasticidin S
・ Blasticidin-S deaminase
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Blasticidin S : ウィキペディア英語版
Blasticidin S

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Blasticidin S is an antibiotic that is produced by ''Streptomyces griseochromogenes''. In biological research, specifically genetic engineering, it is used to select transformed cells which have been engineered to carry a resistance gene for blasticidin. In short, ] of interest is fused to DNA encoding a resistance gene, and then is transformed into cells. After allowing time for recovery and for cells to begin transcribing and translating their new DNA, blasticidin is added. Now only the cells that have the new DNA can grow.
== Resistance genes ==
Three resistance genes have been cloned:
* bls (an acetyltransferase) from ''Streptoverticillum sp.'' which itself produces blasticidin in a natural example of biological warfare
* bsr (a blasticidin-S deaminase) from ''Bacillus cereus'' (other bsr genes are known as well, see listings in Genbank)
* BSD (another deaminase) from ''Aspergillus terreus''
bsr and BSD are the most commonly used resistance genes. The proteins produced from these genes enable the cells carrying them to produce protein in the presence of blasticidin.

抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)
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